TBLASTN 2.6.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: Dm6.32 31,089 sequences; 88,960,239 total letters Query= sp|P08505|IL6_MOUSE Interleukin-6 OS=Mus musculus OX=10090 GN=Il6 PE=1 SV=1 Length=211 ***** No hits found ***** Lambda K H a alpha 0.320 0.135 0.393 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2886574515 Query= sp|P05231|IL6_HUMAN Interleukin-6 OS=Homo sapiens OX=9606 GN=IL6 PE=1 SV=1 Length=212 Score E Sequences producing significant alignments: (Bits) Value FBtr0345011 cdna primary_assembly:BDGP6.32:2R:6972112:6973929:-... 31.6 0.95 FBtr0086185 cdna primary_assembly:BDGP6.32:2R:6972112:6975026:-... 31.6 0.96 FBtr0345802 cdna primary_assembly:BDGP6.32:2R:6971996:6975026:-... 31.6 0.97 FBtr0075330 cdna primary_assembly:BDGP6.32:3L:16845386:16852350... 30.4 2.8 FBtr0084645 cdna primary_assembly:BDGP6.32:3R:24530660:24536395... 29.3 5.9 FBtr0334335 cdna primary_assembly:BDGP6.32:3R:24530576:24536395... 29.3 5.9 > FBtr0345011 cdna primary_assembly:BDGP6.32:2R:6972112:6973929:-1 gene:FBgn0033121 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Cyp6u1 description:Cyp6u1 Length=1760 Score = 31.6 bits (70), Expect = 0.95, Method: Compositional matrix adjust. Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 4/44 (9%) Frame = +1 Query 78 CESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLE 121 C+S K L+E+ N P A + GC SG ++ +GLLE Sbjct 70 CKSRKRQLSEHGSNAPHPAHRPGCAVSGLRPG----QVQSGLLE 189 > FBtr0086185 cdna primary_assembly:BDGP6.32:2R:6972112:6975026:-1 gene:FBgn0033121 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Cyp6u1 description:Cyp6u1 Length=1911 Score = 31.6 bits (70), Expect = 0.96, Method: Compositional matrix adjust. Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 4/44 (9%) Frame = +2 Query 78 CESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLE 121 C+S K L+E+ N P A + GC SG ++ +GLLE Sbjct 221 CKSRKRQLSEHGSNAPHPAHRPGCAVSGLRPG----QVQSGLLE 340 > FBtr0345802 cdna primary_assembly:BDGP6.32:2R:6971996:6975026:-1 gene:FBgn0033121 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Cyp6u1 description:Cyp6u1 Length=2027 Score = 31.6 bits (70), Expect = 0.97, Method: Compositional matrix adjust. Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 4/44 (9%) Frame = +2 Query 78 CESSKEALAENNLNLPKMAEKDGCFQSGFNEETCLVKIITGLLE 121 C+S K L+E+ N P A + GC SG ++ +GLLE Sbjct 221 CKSRKRQLSEHGSNAPHPAHRPGCAVSGLRPG----QVQSGLLE 340 > FBtr0075330 cdna primary_assembly:BDGP6.32:3L:16845386:16852350:1 gene:FBgn0036668 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Zcchc7 description:Zinc finger CCHC-type containing 7 Length=5454 Score = 30.4 bits (67), Expect = 2.8, Method: Compositional matrix adjust. Identities = 19/48 (40%), Positives = 25/48 (52%), Gaps = 0/48 (0%) Frame = +2 Query 51 ERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNLPKMAEK 98 +RID IR++ DGI++L N + KE N N KMAEK Sbjct 4208 KRIDVLIRFLRDGINSLNTNLGNAKSHYVRIKELEGMNTKNGFKMAEK 4351 > FBtr0084645 cdna primary_assembly:BDGP6.32:3R:24530660:24536395:1 gene:FBgn0262167 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ana1 description:anastral spindle 1 Length=5526 Score = 29.3 bits (64), Expect = 5.9, Method: Compositional matrix adjust. Identities = 24/85 (28%), Positives = 39/85 (46%), Gaps = 14/85 (16%) Frame = +1 Query 39 VAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNL------ 92 V A HR +ERI++Q + +D I RK+ +S ++S E N +NL Sbjct 424 VGAAHRAAKEENERIEQQRQERIDKIRRCRKQALKRS--AKASVELRTTNTMNLNAEGRA 597 Query 93 ------PKMAEKDGCFQSGFNEETC 111 P + +K+ +SG N+ C Sbjct 598 SAGTQTPIVEDKENRMESGCNKPCC 672 > FBtr0334335 cdna primary_assembly:BDGP6.32:3R:24530576:24536395:1 gene:FBgn0262167 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:ana1 description:anastral spindle 1 Length=5610 Score = 29.3 bits (64), Expect = 5.9, Method: Compositional matrix adjust. Identities = 24/85 (28%), Positives = 39/85 (46%), Gaps = 14/85 (16%) Frame = +1 Query 39 VAAPHRQPLTSSERIDKQIRYILDGISALRKETCNKSNMCESSKEALAENNLNL------ 92 V A HR +ERI++Q + +D I RK+ +S ++S E N +NL Sbjct 508 VGAAHRAAKEENERIEQQRQERIDKIRRCRKQALKRS--AKASVELRTTNTMNLNAEGRA 681 Query 93 ------PKMAEKDGCFQSGFNEETC 111 P + +K+ +SG N+ C Sbjct 682 SAGTQTPIVEDKENRMESGCNKPCC 756 Lambda K H a alpha 0.315 0.127 0.349 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2913056850 Query= sp|Q0GGL7|IL6_ONCMY Interleukin-6 OS=Oncorhynchus mykiss OX=8022 GN=il6 PE=2 SV=1 Length=219 Score E Sequences producing significant alignments: (Bits) Value FBtr0075282 cdna primary_assembly:BDGP6.32:3L:17049512:17055454... 29.3 5.0 FBtr0075283 cdna primary_assembly:BDGP6.32:3L:17051277:17055454... 29.3 5.0 FBtr0332684 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332681 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332679 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332680 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332678 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332682 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332676 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332675 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332674 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 29.3 7.0 FBtr0332683 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 28.9 7.4 FBtr0332677 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-... 28.9 8.7 > FBtr0075282 cdna primary_assembly:BDGP6.32:3L:17049512:17055454:1 gene:FBgn0036697 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:rogdi description:rogdi Length=2335 Score = 29.3 bits (64), Expect = 5.0, Method: Compositional matrix adjust. Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 1/38 (3%) Frame = +3 Query 166 LSASERKRVLGEVSTGTEWERKTSVHAILRELRNFLVD 203 L+ +ER+ L + EW + VHAIL++LR+ LV+ Sbjct 450 LADTEREEALN-LQIEFEWVLRQEVHAILKQLRSILVE 560 > FBtr0075283 cdna primary_assembly:BDGP6.32:3L:17051277:17055454:1 gene:FBgn0036697 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:rogdi description:rogdi Length=2028 Score = 29.3 bits (64), Expect = 5.0, Method: Compositional matrix adjust. Identities = 15/38 (39%), Positives = 24/38 (63%), Gaps = 1/38 (3%) Frame = +2 Query 166 LSASERKRVLGEVSTGTEWERKTSVHAILRELRNFLVD 203 L+ +ER+ L + EW + VHAIL++LR+ LV+ Sbjct 143 LADTEREEALN-LQIEFEWVLRQEVHAILKQLRSILVE 253 > FBtr0332684 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=46550 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 36940 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 37086 > FBtr0332681 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=47519 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 36940 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 37086 > FBtr0332679 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=48944 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 38365 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 38511 > FBtr0332680 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=49523 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 38944 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 39090 > FBtr0332678 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=55136 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 44557 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 44703 > FBtr0332682 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=55178 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 44599 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 44745 > FBtr0332676 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=55436 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 45826 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 45972 > FBtr0332675 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=56405 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 45826 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 45972 > FBtr0332674 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=56489 Score = 29.3 bits (64), Expect = 7.0, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 45889 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 46035 > FBtr0332683 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=32960 Score = 28.9 bits (63), Expect = 7.4, Method: Compositional matrix adjust. Identities = 18/64 (28%), Positives = 33/64 (52%), Gaps = 5/64 (8%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKDQMKVAE 161 L + KE +E++ L ++L + V EYP + L+P T IG +KD +++ Sbjct 22381 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKDNGELSR 22545 Query 162 VVED 165 +E+ Sbjct 22546 NIEE 22557 > FBtr0332677 cdna primary_assembly:BDGP6.32:3L:2039681:2115611:-1 gene:FBgn0086906 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:sls description:sallimus Length=47528 Score = 28.9 bits (63), Expect = 8.7, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 5/54 (9%) Frame = +1 Query 102 LCSASNKEACLQEISRGLQVYQLLLQHVKAEYPQSTLLPSVTHQTTVLIGLVKD 155 L + KE +E++ L ++L + V EYP + L+P T IG +KD Sbjct 36949 LKPSKRKEKPAEELTVQLPKFRLKARMVLVEYPPAPLIPKTTD-----IGAIKD 37095 Lambda K H a alpha 0.313 0.128 0.361 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3098433195 Database: Dm6.32 Posted date: Feb 21, 2022 12:31 PM Number of letters in database: 88,960,239 Number of sequences in database: 31,089 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40